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Protocols

Protocols

A. Qualitative characteristics

  1. Color parameters (L*,a*, b*, hue angle)
  2. Tissue firmness (Texture analyzer, Chatillon, Effegi)
  3. Soluble solids content
  4. HPLC-Refractive Index determination of sugars
  5. Titratable acidity
  6. HPLC-UV determination of organic acids
  7. Non-destructive determination of fruit maturity stage
  8. Assessment of chilling related disorders with subjective indexes
  9. Dry matter content

B. Cell wall analysis

  1. Cell wall extraction and fractionation
  2. Uronic acid determination
  3. Neutral sugar determination
  4. Specific activity of cell wall modifying enzymes

C. Phytochemical analysis

  1. Determination of total phenolics (Folin-Ciocalteu assay)
  2. Determination of total flavonoids (AlCl3 assay)
  3. Determination of total flavonols (vanillin assay)
  4. Determination of total anthocyanins (pH-differential method)
  5. Determination of reduced ascorbic acid content
  6. Determination of total carotenoids

D. Evaluation of Antioxidant Activity

  1. Radical scavenging activity using DPPH method
  2. Total Antioxidant Activity –FRAP assay
  3. Total Antioxidant Activity –ABTS assay
  4. Evaluation Antioxidant Activity by phosphomolybdenum method
  5. Evaluation Antioxidant Activity by β-carotene bleaching assay

Core Facilities

Core facilities used by the team of Fruit Sciences / PostHarvest Group

  • Fruit Texture Analyzer Titrator
  • F-750 Handheld Brix & Dry Matter Meter (Near Infra-Red Enhanced Spectrometer)
  • DAmeter (Turoni slr)
  • Kiwimeter (Turoni slr)
  • Texture analyzer (TA.XT plus, Stable Micro Systems, Guildford, UK)
  • Digital Refractometer
  • Automatic titrator (DL22 F&B Mettler Toledo)
  • UV-Vis spectrometer
  • Lyophilizer
  • Cas chromatographer (with FID detector)

  • Gas chromatograph/Mass Spectrometer (GCMS-QP2010 Plus GC2010)
  • High pressure liquid chromatography (Waters 1525, Binary HPLC pump, autosampler, UV detector, multi-Wavelength Fluorescence Detector)
  • HPLC-UV-FL (HPLC Liquid High Performance System and Liquid column Temperature Control Oven)
  • Semi-automatic Soxtec extractor (fully semi-automated Soxtec™ 2055 system is used for fast and safe determinations of soluble material in food)
  • Microplate Reader (Model TECAN-INFINITE M200 PRO)

  • FT-IR: FT-IR Shimadzu (IR Prestige 21) the Standard optical bench features a Mid IR measuring range of 7,800 to 350cm-1 , Resolution 0.5,1,2,4,8,16 cm-1(Middle/FarIR) and 2,4,8,16 cm-1(Near IR), signal-to-Noise Ratio Greater than 40,000:1 (4 cm-1 resolution, 1 min accumulation, around 2,100 cm-1, peak-to-peak)
  • FT-NIR: NIR Diode Array 7200 (Perten Instruments): wavelength: 950-1650 nm`, Wavelength accuracy: better than 0.3 nm, detector: T.E. cooled 256 element InGaAs, number of scans: Up to 100 spectra / s, noise:
  • Real Time PCR (Real Time PCR IQ 5 BIO-RAD Thermal Cycler module + Optical Module)

Fruit texture analyzer
Fruit Texture Analyzer
Titrator
Titrator
Devices for non-destructive quality determination
Devices for non-destructive quality determination
Postharvest Unlimited Conference
Chromatometer
Chromatometer
Potentiometric titrator
Potentiometric titrator
Refractometer
Refractometer
Texture analyzer
Texture Analyzer
Fruit firmness tester
Fruit firmness tester
Sinclair IQ firmness tester
Sinclair IQ firmness tester
Solid Phase Extraction
Solid Phase Extraction
Soxtec extractor
Soxtec extractor
Sinclair IQ firmness tester
Sinclair IQ firmness tester
HPLC
HPLC
FT-IR
FT-IR
Tecan Micro-plate Reader
Tecan Micro-plate Reader
Freeze dryer
Freeze dryer
Rotary evaporator
Rotary evaporator

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